Coding

Part:BBa_K3096023

Designed by: Lina Widerspick, Famke Baeuerle, Patrick Mueller, Jakob Keck   Group: iGEM19_Tuebingen   (2019-10-14)

GFP with linkers for fusion with His Tag and CPPs

This part is a GFP with linkers to be fused to His Tag and CPPs. It is incorporated in all composite CPP parts designed by iGEM Tübingen 2019 to allow for the intracellular detection of transported cargo.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 721
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 650


[edit]
Categories
Parameters
None